Plant Transcription Factor Database
Previous version: v3.0
Micromonas sp. RCC299
C3H Family
Species TF ID Description
100424C3H family protein
108666C3H family protein
108954C3H family protein
56045C3H family protein
56076C3H family protein
56416C3H family protein
56710C3H family protein
56817C3H family protein
57579C3H family protein
59514C3H family protein
59897C3H family protein
60445C3H family protein
60560C3H family protein
61222C3H family protein
62104C3H family protein
62171C3H family protein
63899C3H family protein
64358C3H family protein
64506C3H family protein
70339C3H family protein
79899C3H family protein
99494C3H family protein
C3H Family Introduction

One gene isolated by virtual subtraction is PEI. It encodes a protein containing a Cys3His zinc finger domain associated with a number of animal and fungal transcription factors. In situ hybridization results showed that PEI1 is expressed throughout the embryo from globular to late cotyledon stage. Transgenic Arabidopsis plants expressing a PEI1 antisense gene produced white seeds in which embryo development did not progress through heart stage. Aberrant embryos failed to form cotyledons, but the embryonic root appeared to be normal. Aberrant embryos did not turn green, and the expression of genes involved in photomorphogenesis was drastically attenuated. In culture, aberrant embryos did not form true leaves, but root formation was apparently normal. These results suggest that PEI1 is an embryo-specific transcription factor that plays an important role during Arabidopsis embryogenesis, functioning primarily in the apical domain of the embryo.

we used a random binding site selection method to show that recombinant PEI1 protein purified from Escherichia coli can bind to specific DNA sequences. This DNA binding activity supports the prediction that PEI1 is an embryo-specific transcription factor.

Z Li and T L Thomas.
PEI1, an embryo-specific zinc finger protein gene required for heart-stage embryo formation in Arabidopsis.
Plant Cell, 1998. 10(3): p. 383-98.
PMID: 9501112